human serum proteins  (Jackson Immuno)

Journal: bioRxiv

Article Title: Denatured Albumin Gains a Function of Regulating Platelet Activity

doi: 10.64898/2026.03.30.715112

Figure Lengend Snippet: (A) Platelets (green) adhered exclusively to the albumin-adsorbed region (magenta), but not to the casein-adsorbed region (dark). Albumin was pre-labeled with Alexa488. Platelets were stained with phalloidin to label F-actin. (B) Resting platelets, ADP-activated platelets and HeLa cells on fibronectin (FN), fibrinogen (Fg), bovine serum albumin (BSA), human serum albumin (HSA), recombinant human serum albumin (rHSA) and casein-adsorbed glass surfaces, respectively. ( C-E ) Cell spreading areas of platelets or HeLa cells on the six surfaces. Platelets in (C) were kept at a resting state prior to cell plating, whereas platelets in (D) were pre-activated by 10 µM ADP. (**** P <0.0001; ns P >0.05; Each data point represents one cell; For each condition, N = 100 cells from three independent experiments; Error bars indicate SD).

Article Snippet: Activated platelets or resting platelets at concentration of 4.0 × 10 4 /μL were seeded onto glass coverslips pre-coated with 10 μg/ml fibronectin (F1141, Sigma-Aldrich), 100 μg/mL fibrinogen (F3879, Sigma-Aldrich), 100 μg/mL ultrapure bovine serum albumin (AM2616, Invitrogen), 100 μg/mL ultrapure human serum albumin (A8763, Sigma-Aldrich), 100 μg/mL recombinant human serum albumin (HSA-H5220, Acro Biosystems) or 100 μg/ml casein, and incubated at 37 °C in a cell incubator containing 5% CO2 for 30 minutes.

Techniques: Labeling, Staining, Recombinant

Journal: Cell metabolism

Article Title: Cytoarchitectural multi-depot profiling reveals immune-metabolic crosstalk in human colon-associated adipose tissue

doi: 10.1016/j.cmet.2025.12.008

Figure Lengend Snippet: (A) Cell-cell communication analysis of SAA signaling pathway for the indicated depots and cells. Line colors and widths represent the sending cell and the strength of the signal, respectively. (B) Pseudobulk comparison of differentially expressed genes in epiploic vs. subcutaneous depots, highlighting the indicated signaling pathways. (C) Expression of SAA1 mRNA upon stimulation with LPS (10 ng/mL), TNF-α (2.5 ng/mL), IL-1β (10 ng/mL), or IL-6 (10 ng/mL) for 24 h. Data are presented as fold change (FC) over vehicle. Significant (<0.05) p values compared with the vehicle from one-way ANOVA are shown. (D) Relative expression of SAA1 (left) and SAA2 (right) mRNA in adipocytes, ASPCs, adipose-derived endothelial cells (adECs), and THP1-derived macrophages (THP1 M0). Significant (<0.05) p values compared with the cell-type-specific control from one-way ANOVA are shown. (E) Results from ELISA measurements of SAA in conditioned media from human adipocytes incubated without or with LPS for 72 h. Results are displayed as mean, error bars represent standard error of the mean, and statistical significance was calculated using Student’s t test. (F) Analyses of DNA methylation in the indicated cell classes for the promoter and gene body regions of SAA1 and SAA2 , respectively. Chromosomal localization and motifs for NFKB2/RELA/REL and STAT3 are indicated for both genes. (G) SAA1 and SAA2 mRNA expression in adipocytes treated with or without LPS in the presence or absence of different inhibitors described in the main text. Data are presented as FC over vehicle. Significant (<0.05) p values compared with the vehicle from one-way ANOVA are shown. For (C), (D), and (G), data are displayed as geometric mean ± 95% confidence intervals with technical replicates displayed from three independent experiments. ASPC, adipose stromal and progenitor cell.

Article Snippet: Human recombinant SAA1 protein , OriGene , Cat#TP310664.

Techniques: Comparison, Protein-Protein interactions, Expressing, Derivative Assay, Control, Enzyme-linked Immunosorbent Assay, Incubation, DNA Methylation Assay

Journal: Cell metabolism

Article Title: Cytoarchitectural multi-depot profiling reveals immune-metabolic crosstalk in human colon-associated adipose tissue

doi: 10.1016/j.cmet.2025.12.008

Figure Lengend Snippet: (A) Top Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways based on differentially expressed genes from comparisons of SAA1- or LPS-treated vs. control-treated adipose-derived stromal-vascular cells. (B) Network view of the six common SAA1- and LPS-regulated pathways showing shared and treatment-specific genes. (C) Expression scores for SAA1- (left) and LPS-responsive (right) genes following a 6-h incubation with either ligand in stromal-vascular cells from subcutaneous human WAT. Results are displayed on the uniform manifold approximation and projection panel from and violin plots for each indicated cell class. Note that adipocytes and mesothelial cells were removed as these are not present in the stromal-vascular fraction from the subcutaneous depot. (D) Representative immunostaining of epiploic adipose tissue using antibodies directed against markers for Adipo SAA (CES1) and macrophages (CD68). Hoechst33342 was used as the counterstain for nuclei. Scale bar, 100 μm. ASPC, adipose stromal and progenitor cell; NES, normalized enrichment score.

Article Snippet: Human recombinant SAA1 protein , OriGene , Cat#TP310664.

Techniques: Control, Derivative Assay, Expressing, Incubation, Immunostaining